Metabolomic analysis by UAE-GC MS and antioxidant activity of <i>Salvia hispanica </i>(L.) seeds grown under different irrigation regimes

Chia (Salvia hispanica L.) is an emerging crop with a high content of α-linolenic acid and metabolites of industrial and pharmaceutical interest but information on metabolome variations in response to agricultural management is scarce. We investigated the yield and metabolic profile of the seeds of two chia populations, one commercial black (B) and one long-day flowering genotype (G8), in response to two irrigation levels: replacement of 100% ET⁠0 (I) or rainfed (NI). Seed yield was higher in irrigated plots in G8 only (0.255kgm⁠−2 for I vs 0.184kgm⁠−2 for NI) while it was very low regardless of irrigation in B due to late flowering. Ultrasound assisted extraction (UAE) of seeds followed by gas chromatography-mass spectrometry (GC/MS) analysis showed differences in fatty acids and the major classes of organic compounds due to both genotype and irrigation, especially in the non-polar phase where irrigated samples showed a higher content of α-linolenic and other fatty acids and a lower oleic/linoleic ratio (47.4 in NI vs. 39.6 in I). The antioxidant activity, expressed as trolox equivalent antioxidant capacity (TEAC), ranged from 1.317±0.027 to 2.174±0.010mmol TEAC/g of defatted chia seed after 2 and 40min respectively, and was negatively affected by irrigation. The total polyphenolic content (TPC) measured with the Folin-Ciocalteu method, also decreased with irrigation. According to our results irrigation can affect chia yield, metabolome and antioxidant behavior but some of the effects are genotype-dependent

Una heurística de derivación de LEL a escenarios

El objetivo principal de este trabajo de tesis está centrado en derivar escenarios contribuyendo y enriqueciendo las estrategias existentes. Utilizaremos la información del LEL que aún no ha sido tenido en cuenta, con el propósito de generar escenarios más completos que describan mejor los requerimientos funcionales.Facultad de Informátic

Metabolomic analysis and antioxidant activity of wild type and mutant Chia (Salvia hispanica L.) stem and flower grown under different irrigation regimes

BACKGROUND: Chia (Salvia hispanica L.) is a functional food from Central America. Interest in it is growing rapidly due to the many health benefits from the seed. However, when chia is grown at high latitudes, seed yield may be low whereas a high stem biomass and immature inflorescences are produced. Little is known about the chemical composition and the properties of stems and flowers. In this work, the metabolite profile, the antioxidant activity, and the total polyphenol content of stems and inflorescences were evaluated in a factorial experiment with different chia populations (commercial black chia and long-day flowering mutants G3, G8, and G17) and irrigation (100% and 50% of evapotranspiration).RESULTS: The results show the influence of irrigation and seed source on the antioxidant activity and total polyphenol content of chia flower and stem. Inflorescences exhibit higher antioxidant activity, suggesting their potential use as natural antioxidant. The mutants G3 and G8, at 50% irrigation, contained the highest amounts of compounds with nutraceutical value, especially within the flower. The mutant G17 showed lower antioxidant activity and polyphenol content compared to other seed sources but exhibited high omega 3 content in flowers but low in stems. This indicates that chia varieties should be chosen according to the objective of cultivation.CONCLUSION: These findings, indicating a close relation of metabolite content with irrigation and seed source, may provide the basis for the use of chia flower and stem for their nutraceutical value in the food, feed, and supplement industries.</p

Lifetime use of psychedelics is associated with better mental health indicators during the COVID-19 pandemic

Background and aimsThe COVID-19 pandemic and its consequences represent a major challenge to the mental health and well-being of the general population. Building on previous work on the potential long-term benefits of psychedelics, we hypothesized that lifetime use of these drugs could be linked to better mental health indicators in the context of the ongoing pandemic.MethodsTwo anonymous online surveys were conducted between April and June 2020, including questions about lifetime experience with psychedelics and other psychoactive drugs, and psychometric scales designed to measure personality traits, anxiety, negative, and positive affect, well-being, and resilience. Principal component analysis was applied to divide the sample into groups of subjects based on their drug use reports.ResultsFive thousand six hundred eighteen participants (29.15 ± 0.12 years, 71.97% female) completed both surveys and met the inclusion criteria, with 32.43% of the sample reporting at least one use of a psychedelic drug. Preliminary analyses showed that certain psychedelics were linked to improved mental health indicators, while other psychoactive drugs exhibited the opposite behavior. Lifetime psychedelic use was linked to increased openness and decreased conscientiousness, and to higher scores of positive affect. The reported number of past psychedelic experiences predicted higher scores of the secondary personality trait beta factor, which has been interpreted as a measure of plasticity. No significant associations between lifetime use of psychedelics and indicators of impaired mental health were observed.ConclusionWe did not find evidence of an association between lifetime use of psychedelics and poor mental health indicators. Conversely, experience with psychedelic drugs was linked to increased positive affect and to personality traits that favor resilience and stability in the light of the ongoing crisis.Fil: Cavanna, Federico Amadeo. Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; ArgentinaFil: Pallavicini, Carla. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina. Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia; ArgentinaFil: Milano, Virginia. No especifíca;Fil: Cuiule, Juan. No especifíca;Fil: Di Tella, Rocco. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; ArgentinaFil: González, Pablo. No especifíca;Fil: Tagliazucchi, Enzo Rodolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina. Universidad Adolfo Ibañez; Chil

Cr(III) Complexes Bearing a β-Ketoimine Ligand for Olefin Polymerization: Are There Differences between Coordinative and Covalent Bonding?

β-ketoimines are extensively applied for the synthesis of organometallic complexes intended as (pre)catalysts for a variety of chemical transformations. We were interested in the synthesis of two Cr complexes bearing a simple bidentate β-ketoimine (L), with different ligand binding modes, as well as their application as a precatalyst in the polymerization of olefins. Complex 1 (L2CrCl3) was obtained by direct reaction of L with CrCl3(THF)3, while, for the synthesis of complex 2 (LCrCl2), the ligand was first deprotonated with nBuLi, giving the β-ketoiminato ligand L─Li+, and then reacted with CrCl3(THF)3. Characterization of the complexes proved that the Cr(III) ion is coordinatively bonded to L in 1, while it is covalently bonded to L in 2. The complexes were then used as precatalysts for the polymerization of ethylene and various cyclic olefins. Upon activation with methylaluminoxane, both the complexes exhibited poor activity in the polymerization of ethylene, whilst they exhibit good productivity in the polymerization of cyclic olefins, affording semicrystalline oligomers, without a significant difference between 1 and 2. To gain more insight, we investigated the reaction of the complexes with the Al-cocatalyst by IR and UV-Vis spectroscopies. The results proved that, in case of 1, the Al-activator deprotonates the ligand, bringing to the formation of an active species analogous to that of 2

Simultaneous presentation of Waldenström macroglobulinemia and multiple myeloma: multidisciplinary diagnosis, treatment and 30-month follow-up.

Waldenström macroglobulinemia and multiple myeloma are mature B-cell neoplasms deriving from post-germinal cells at different stages of differentiation. The simultaneous presentation of Waldenström macroglobulinemia and multiple myeloma in the same patient is a very rare phenomenon and, so far, only two cases have been described. We report the case of a 75-year Caucasian female patient, with a silent clinical history, who presented with anemia and two different monoclonal proteins (IgMκ and IgGκ). The trephine biopsy showed the presence of a dual population, represented by small lymphoplasmacytoid cells and by plasma cells, which infiltrated the bone marrow with a clearly different pattern. Both immunohistochemistry and flow cytometry demonstrated the biclonal origin such neoplastic cells, since lymphoplasmacytoid cells resulted IgMκ while plasma cells were IgGκ. This biclonal pattern was further confirmed by the demonstration of a different IgH gene rearrangement of the two neoplasms. The patient was treated with bortezomib, dexamethasone and rituximab, achieving partial remission of both Waldenström macroglobulinemia and multiple myeloma. After a 30-month follow-up, she is in stable disease. Multiple myeloma has been described in association with other indolent B-cell neoplasms, mostly chronic lymphocytic leukemia, while Waldenström macroglobulinemia can be followed by diffuse large B-cell lymphoma in some instances, after chemotherapy. The association of Waldenström macroglobulinemia and multiple myeloma seems to be very rare. Our study shows that an integrated diagnostic work-up is very useful in such cases, with an interesting role for flow cytometry. [J Clin Exp Hematop 53(1): 29-36, 2013]

Desenvolvimento e caracterização de painéis de partículas aglomeradas utilizando o resíduo do ouriço da Castanha-do-Brasil (Bertholletia excelsa) e resina poliuretana derivada do óleo da mamona

O presente trabalho teve como objetivo avaliar a viabilidade da produção de painéis de partículas aglomera-das utilizando-se o resíduo obtido após o beneficiamento do ouriço da Castanha-do-Brasil (Bertholletia ex-celsa) associado à resina poliuretana derivada do óleo da mamona. Um estudo das variáveis granulometria das partículas e quantidade de resina foi realizado, com base em um planejamento experimental 22+1. A qua-lidade dos painéis produzidos foi avaliada com base nas prescrições do documento normativo ABNT NBR 14810:2 (2002), por meio de ensaios de absorção de umidade (2 e 24h), inchamento em espessura (2 e 24h) e arrancamento de parafuso (topo e superfície), adicionalmente, realizou-se um ensaio de dureza Janka e análi-se do teor de cinzas. Com os resultados obtidos, foi possível verificar que a incorporação de resíduo do ouri-ço da Castanha-do-Brasil apresenta potencial na produção de painéis de partículas aglomeradas, e que a adi-ção de resina poliuretana à base do óleo da mamona mostrou-se eficiente como adesivo aglomerante, indi-cando que maiores porcentagens de resina tendem a implicar em diminuição da absorção de água e variação dimensional dos painéis, assim como aumento da resistência mecânica.Palavras-chave: Painéis de partículas, resíduos lignocelulósicos, poliuretano natural

Arte gráfico activo

Tomamos para el desarrollo del presente escrito una obra de Marcel Duchamp y a través de sus líneas conceptuales revisamos los tópicos del arte contemporáneo, a la vez que los implementamos de manera teórica y práctica en la enseñanza de arte en el taller de grabado complementario. Concebimos la producción de la teoría como una práctica activa del arte y en este repensar se construye la obra con el otro alumno. Obras implican un sinnúmero de acciones y procesos de construcciones sensibles que incluyen las charlas, las estrategias de abordaje, las decisiones tomadas, las construcciones colectivas, lo afectivo puesto en juego, la apertura a nuevas redes y los vínculos comunitarios, es ahí donde detectamos la gráfica activa.Facultad de Bellas Artes (FBA

Arte gráfico activo

Tomamos para el desarrollo del presente escrito una obra de Marcel Duchamp y a través de sus líneas conceptuales revisamos los tópicos del arte contemporáneo, a la vez que los implementamos de manera teórica y práctica en la enseñanza de arte en el taller de grabado complementario. Concebimos la producción de la teoría como una práctica activa del arte y en este repensar se construye la obra con el otro alumno. Obras implican un sinnúmero de acciones y procesos de construcciones sensibles que incluyen las charlas, las estrategias de abordaje, las decisiones tomadas, las construcciones colectivas, lo afectivo puesto en juego, la apertura a nuevas redes y los vínculos comunitarios, es ahí donde detectamos la gráfica activa.Facultad de Bellas Artes (FBA

Arte gráfico activo

Tomamos para el desarrollo del presente escrito una obra de Marcel Duchamp y a través de sus líneas conceptuales revisamos los tópicos del arte contemporáneo, a la vez que los implementamos de manera teórica y práctica en la enseñanza de arte en el taller de grabado complementario. Concebimos la producción de la teoría como una práctica activa del arte y en este repensar se construye la obra con el otro alumno. Obras implican un sinnúmero de acciones y procesos de construcciones sensibles que incluyen las charlas, las estrategias de abordaje, las decisiones tomadas, las construcciones colectivas, lo afectivo puesto en juego, la apertura a nuevas redes y los vínculos comunitarios, es ahí donde detectamos la gráfica activa.Facultad de Bellas Artes (FBA